HR |
Expert advisor
Department of Dairy Science
Pavilion 2nd, 1st floor
tel: +385 01 239 3904
e-mail: jspoljaric@agr.hr
Research interest
Analytical methods of milk and dairy products
Academic qualifications
2010 – Dissertation
University of Zagreb - Faculty of Science
Metallopeptydase family M49: heterologous expression, purification and characterization of an human orthologous enzyme.
2003 – Master's Thesis
University of Zagreb - Faculty of Science, course: Chemistry (biochemistry)
Proline-specific aminopeptidase from Streptomyces rimosus.
1998 – Graduate work
University of Zagreb - Faculty of Food Technology and Biotechnology, course: Biochemical engineering
Preparation of desulfoglucosinolate (DSGSL) from cabbage for reverse phase liquid chromatography (RPLC).
Work experience
May, 2011 – now
University of Zagreb - Faculty of Agriculture
Department of Dairy Science
Reference Laboratory for Milk and Dairy Science
October, 1999 – April, 2011
“Ruđer Bošković” Institute, Zagreb, Croatia
Department for Organic Chemistry and Biochemistry
Laboratory of Cellular Biochemistry
EduCATION
2012
40th Croatian dairy experts symposium with international participation, Lovran, Croatia, Croatian Dairy Union (HMU).
Seminar “Application of norm HRN EN ISO/IEC 17025, Zagreb, Croatia, Croatian Metrology Society (HMD).
2011
Seminar “Waters – UPLC Instruments”, Zagreb, Croatia, Biotehnika.
Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology in Osijek, Department of Applied Chemistry and Ecology, Laboratory of Biochemistry.
2010
Institute of Biochemistry, Graz, Austria.
2004
Pre-conference Tutorial “Modern Methods of Mass Spectrometry in Chemistry and Biology”, 25th September, Seggau, Austria.
“Introduction to Bioinformatics” (attended the Practical Course) Zagreb, Croatia, Faculty of Science, University of Zagreb.
2000
7th International Summer School on Biophysics “Supramolecular Structure and Function”, Rovinj, Croatia, Croatian Biophysical Society.
Projects
2012 – “Quality control of cheeses on the market in order to increase competitiveness”, Ministry of Agriculture (VIP), Jasmina Havranek, University of Zagreb Faculty of Agriculture.
2007–2011, “Molecular enzymology and protein interactions of hydrolases”. Ministry of Science, Education and Sport. Marija Abramić, Ruđer Bošković Institute.
2002–2005, “Hydrolases - from isolation to function”. Ministry of Science, Education and Sport. Marija Abramić, Ruđer Bošković Institute.
Scientific and professional papers
Vukelić, B., Salopek-Sondi, B., Špoljarić, J., Sabljić, I., Meštrović, N., Agić, D., Abramić, M. (2012) Reactive cysteine in the active-site motif of Bacteroides thetaiotaomicron dipeptidyl peptidase III is a regulatory residue for the enzyme activity. Biological Chemistry. 393. (accepted for publication)
Havranek, J., Mikulec, N., Gagić, R., Antunac, N., Špoljarić, J., Kalit, S. (2011) The adventages and disadvantages of traditional production as opposed to highly sophisticated industrial production of cheese. International Conference on Functional Dairy Foods. 6-7, Karnal, India.
Špoljarić, J., Tomić, A., Vukelić, B., Salopek-Sondi, B., Agić, D., Tomić, S., Abramić, M. (2011) Human dipeptidyl peptidase III: The role of Asn406 in ligand binding and hydrolysis. Croatica Chemica Acta. 84(2), 259-268.
Tomić, A., Abramić, M., Špoljarić, J., Agić, D., Smith, D. M., Tomić, S. (2011) Human dipeptidyl peptidase III: Insights into ligand binding from a combined experimental and computational approach. Journal of Molecular Recognition. 24(5), 804-814.
Špoljarić, J., Salopek-Sondi, B., Makarević, J., Vukelić, B., Agić, D., Šimaga, Š., Jajčanin-Jozić, N., Abramić, M. (2009) Absolutely conserved tryptophan in M49 family of peptidases contributes to catalysis and binding of competitive inhibitors. Bioorganic Chemistry. 37(1/3), 70-76.
Špoljarić, J., Vukelić, B., Vitale, Lj. (2009) Aminopeptidases in mycelium and growth medium of Streptomyces rimosus strains. Food Technology and Biotechnology. 47(3), 288-295.
Salopek-Sondi, B., Vukelić, B., Špoljarić, J., Šimaga, Š., Vujaklija, D., Makarević, J., Jajčanin, N., Abramić, M.(2008) Functional tyrosine residue in the active center of human dipeptidyl peptidase III. Biological Chemistry. 389(2), 163-167.
Baršun, M., Jajčanin, N., Vukelić, B., Špoljarić, J., Abramić, M. (2007) Human dipeptidyl peptidase III acts like a post-proline-cleaving enzyme on endomorphins. Biological Chemistry. 388, 343-348.
Abramić, M., Špoljarić, J., Šimaga, Š. (2004) Prokaryotic homologs help to define consensus sequences in peptidase family M49. Periodicum Biologorum. 106(2), 161-168.
Vukelić, B., Schroeder, W., Špoljarić, J., Vitale, Lj. (2004) Deoxyribonuclaese from Streptomyces rimosus correlates with hypothetical proteins from Streptomyces coelicolor and Streptomyces avermitilis. Periodicum Biologorum. 106(2), 141-145.
